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A novel fructosyltransferase(AoFT)capable of catalyzing the synthesis of sucrose-6-acetate(S-6-a)from sucrose and glucose-6-acetate has been purified to homogeneity from Aspergillus oryzae ZZ-01 by ammonium sulfate precipitation,Q sepharose,phenyl sepharose and sephacryl S-200 HR chromatography.