【摘 要】
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There are 50(24.6％)strains carrying qnrD in 203 Proteeae,and 16 strains were found to be co-produced other PMQR genes,low-level fluoroquinolone resistance was exhibited by most isolates with qnrD.Sout
【机 构】
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温州医科大学附属第一医院 325000
【出 处】
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中华医学会第十一次全国临床微生物学术年会、全球华人临床微生物与感染症学会学术论坛暨第四届国际临床微生物及抗微生物化疗学术
论文部分内容阅读
There are 50(24.6％)strains carrying qnrD in 203 Proteeae,and 16 strains were found to be co-produced other PMQR genes,low-level fluoroquinolone resistance was exhibited by most isolates with qnrD.Southern blot analysis of the qnrD-positive isolates confirmed that qnrD was encoded on the ～2.7-kb or ～5.2-kb plasmid.Conjugal studies proved that all the isolates failed to transfer qnrD gene to recipient.But the plasmids carrying qnrD were successfully transferred by transduction,and transformants showed 62.5-to 300-fold increases in the MICs of quinolones,which proved qnrD contributed to quinolone resistance and suggested that the qnrD-carrying plasmids could spread by natural transformation and transduction.PFGE showed that these isolates were divergent,this study provided evidence for the frequent association of qnrD with the Proteeae.We hypothesized that qnrD could be originated from Proteeae.qnrD can transfer and spread if the plasmid acquired mobile elements.
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