【摘 要】
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The CD40 ligand(CD40L)has shown potential as a powerful immunological adjuvant in various studies.Here,the efficacy of a chimeric subunit vaccine,consisting of Eimeria tenella immune mapped protein 1(
【机 构】
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Engineering Laboratory of Animal Pharmaceuticals and College of Animal Science,Fujian Agriculture an
【出 处】
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中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
论文部分内容阅读
The CD40 ligand(CD40L)has shown potential as a powerful immunological adjuvant in various studies.Here,the efficacy of a chimeric subunit vaccine,consisting of Eimeria tenella immune mapped protein 1(EtIMP1)and chicken CD40L,was evaluated against E.tenella infection.The recombinant EtIMP1-CD40L was purified from E.coli over-expressing this protein.Chickens were vaccinated with EtIMP1-CD40L without adjuvant or EtIMP1 with Freunds adjuvant.Immunization of chickens with EtIMP1-CD40L fusion protein resulted in stronger IFN-γ secretion and IgA response than that with only recombinant EtIMP1 with Freunds adjuvant.The clinical effect(cecal lesions,body weights gain,and oocysts shedding)of the EtIMP1-CD40L without adjuvant was also better than that of the EtIMP1 with adjuvant,as evidenced by the difference between the two groups in the oocyst output of E.tenella-ehallenged chickens.The results suggest that the EtIMP1-CD40L fusion protein can be used as an effective immunogen in the development of subunit vaccines against Eimeria infection.
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