Natural and synthetic congeners of carbazole comprise a large group of therapeutically useful agents in medicinal chemistry.Herein a series of novel isopropanol-bridged sulfonyl carbazoles as novel potential antimicrobial agents were designed and synthesized via multi-step procedure from commercial aniline and carbazole in an effort to overcome drug resistance.Most of the prepared compounds exerted powerful antibacterial activities toward a panel of Gram-positive and Gram-negative organisms,including drug-resistant pathogens.The active molecules could effectively intercalate into methicillin-resistant Staphylococcus aureus DNA via supramolecular interactions,which might block DNA replication to exhibit their powerful antibacterial activity.