Objective It is known that aminoglycosides can promote readthough of nonsense codons to produce full-length proteins in many genetic diseases caused by nonsense mutation.Recently, PTC124 with low toxicity has been indicated as a new compound for the treatment of nonsense mutations.The purpose of this study was to explore the effect of three different aminoglycosides sharing a 4, 6-disubstituted ring and PTC 124 on rescuing nonsense mutations of HERG gene associated with LQT2 syndrome.