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Speciation analysis of chromium has been carried out using HPLC and ICP-MS in tandem.Chromium containing compounds,including Cr(III)and Cr(VI),were well separated by reversed phase HPLC with a C8-HPLC column as the stationary phase and 0.5 mmol L-1 tetrabutyl ammonium phosphate(TBAP)and 0.3 mmol L-1 EDTA solution(pH 6.9)as the mobile phase using isocratic elution.The chromatographic separation was complete in less than 110 s.