CuO microspheres were directly grown on Ni foam via one-pot hydrothermal method and designed to construct a hybrid electrode for an amperometric non-enzymatic glucose sensor.The scanning electron microscopy(SEM)and X-ray diffraction(XRD)were used to characterize the morphologies and structures,the SEM images showed that large amounts of spherical nanoparticles were well dispersed on the surface of Ni foam and XRD indicated the CuO crystalline phase.The direct electrocatalytic oxidation of glucose in alkaline medium at CuO microspheres modified electrodes has been investigated in detail using cyclic voltammetry and chronoamperometry demonstrate its performance for glucose detection.The electrode displayed a synergistic effect of copper oxide nanoparticles and Ni foam towards the oxidation of glucose,showing a considerable oxidation current at +0.4 V.The CuO/Ni foam hybrid electrode exhibits a sensitivity of 6.89 mA mM-1 cm-2 with a detection limit of 10 mM(S/N=3),and the response time is 3s.As a result,for amperometric non-enzymatic glucose detection the microspheres CuO-modified Ni foam electrode exhibits high sensitivity,low detection limit and good stability to construct a promising nonenzymatic glucose sensor.