激活标签法初步构建甘蓝型油菜(Brassica napus)突变体库

来源 :2013全国植物生物学大会 | 被引量 : 0次 | 上传用户：ppc8xzf

【摘要】

：

　　目前，在拟南芥中已经成功建立了接近饱和的插入突变体库，水稻中获得的插入标签侧翼序列已经超过了两万个，而且这一数据还在不断增长。然而，有关油菜T-DNA插入突变体库的研究

【作者】

：

黄琪郑香峰马忠岩谭小力

【机构】

：

江苏大学生命科学研究院,江苏镇江212013

【出处】

：

2013全国植物生物学大会

【发表日期】

：

2013年10期

【关键词】

：

突变体库甘蓝型油菜激活标签法

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　　目前，在拟南芥中已经成功建立了接近饱和的插入突变体库，水稻中获得的插入标签侧翼序列已经超过了两万个，而且这一数据还在不断增长。然而，有关油菜T-DNA插入突变体库的研究却少有报道。我们利用激活标签技术，在T-DNA的一端放置4个强启动子。当这种T-DNA插入基因的上游时会致该基因组成型过量表达；当插入基因中间时，就会干扰插入位点基因的正常表达。

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激活标签法初步构建甘蓝型油菜(Brassica napus)突变体库

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