Methods of production of dried probiotic powders should be such that viability is maintained in the dried powders following manufacture,and storage to ensure that an adequate number of bacteria can be delivered in the final product.This work presents a study of the spray drying process of an autochthonous strain of Lactobacillus acidophilus isolated by the research group from a healthy woman from the BioBio Region,Chile,in order to enhance the cells viability and probiotics properties during storage.Identification of Lactobacillus strain was performed by Gram reaction,biochemical profiles and the API CH50 System.The results show that the powder obtained presents dried strain viability in the range of 10+8-10+9 (CFU/g)with product moisture content below 10%.