Skeletal tissue has the capability to adapt its mass and structure in response to mechanical stress.However,the molecular mechanism of bone and cartilage to respond to mechanical stress are not fully understood.A label-free quantitative proteome approach was used for the first time to obtain a global perspective of the response of skeletal tissue to mechanical stress.Label-free quantitative analysis of 1D-PAGE-LC/MS/MS based proteomics was applied to identify differentially expressed proteins.