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5. AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discove

AFSM sequencing approach: a simple and rapid method for genome-wide SNP and methylation site discove

来源 :第十六届全国植物基因组学大会 | 被引量 : 0次 | 上传用户：mulan0924

【摘 要】

：

　　We describe methods for the assessment of amplified-fragment single nucleotide polymorphism and methylation (AFSM) sites using a quick and simple molecular

【作 者】

：

ZhiqiangXia;MeilingZou;ShengkuiZhang;BinxiaoFeng;WenquanWang; 

【机 构】

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The Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agriculture Sci

【出 处】

：

第十六届全国植物基因组学大会

【发表日期】

：

2015年期

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论文部分内容阅读

　　We describe methods for the assessment of amplified-fragment single nucleotide polymorphism and methylation (AFSM) sites using a quick and simple molecular marker-assisted breeding strategy based on the use of two restriction enzyme pairs (EcoRI-MspI and EcoRI-HpaⅡ) and a next-generation sequencing platform.

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