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Aim Forsythia suspensa (Thunb.) Vahl, Lianqiao in Chinese, is one of the most fundamental herbs in traditional Chinese medicine (TCM) with heatclearing and detoxicating properties.In this study, we aimed to study the antitumor activity of Lianqiao aqueous extract against melanoma using cancer cell linebased in vitro and mouse allograft tumor in vivo models.Furthermore, we also investigated the underlying molecular mechanisms, particularly the involvement of antiinflammation and antioxidation properties in its antitumor activity.Methods The proliferation of cancer ceils was measured by MTT assay.The transplanted B16F10 melanoma in C57BL/6 mice were established and used for the evaluation of in vivo antitumor effect of LQ.Tumor growth was monitored twice a week.Ki67 and CD31 were used to detect cancer cell proliferation and angiogenesis in tumor, respectively.The antioxidative property of LQ was determined by measuring the levels of ROS, MDA and GSH.The antiinflammatory effect of LQ was evaluated by measuring TNFα and IL6 using ELISA kits.Other protein expression was determined by Western Blot.Results LQ strongly inhibited the growth of B16F10 cells in vitro and the tumor growth in vivo.The survival time of tumorbearing mice was significantly prolonged by LQ.LQ inhibited cancer cell proliferation and angiogenesis in tumor as evidenced by decreased expressions of Ki67 and CD31.Levels of ROS, MDA TNFα and IL6 decreased, while GSH increased in LQ treatment group, indicating a strong antioxidative and antiinflammatory activity of LQ.The expression of antioxidant proteins Nrf2 and HO1, tumor suppressors P53 and pPTEN, and the MAPK pathways in tumor tissues were upregulated by LQ treatment.Conclusions LQ exhibited strong antitumor activity against B16F10 murine melanoma both in vitro and in vivo.The antitumor effect of LQ involved the decreased oxidative stress and inflammation in tumor, which is closely related to the heatclearing and detoxicating properties of LQ.