家蚕核型多角体病毒泛素修饰靶标的鉴定

来源 :第十一届全国病毒学学术研讨会暨第六届武汉现代病毒学国际研讨会 | 被引量 : 0次 | 上传用户:a272437762
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  家蚕核型多角体病毒(Bombyx mori nucleopolyhedrovirus,BmNPV)是能够自身编码泛素基因的杆状病毒属病毒成员,而泛素-蛋白酶体系统在BmNPV的早期复制过程中扮演了重要角色,所以在BmNPV侵染过程中,病毒及宿主泛素化蛋白的鉴定对于研究泛素-蛋白酶体系统在病毒复制中所起得作用显得尤为重要。本研究借助Red重组和杆状病毒表达系统构建出只融合表达泛素-myc-his蛋白的重组病毒,通过镍住纯化获得泛素化蛋白,最终利用LC-MS/MS技术对这些蛋白进行了鉴定。
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