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Aims：The goal of cell transcription for treatment of diabetes is to generate surrogateβ-cells from an appro—pilate cell line.However,the induced replacement cells have showed less physiological function in producing in—sulin compared with normal β-cells.Methods：Here,we report a procedure for induction of insulin-producing.cells(IPCs)from bone ma/Tow murine mesenehymal stem cells(BM-mMSCs).These BM-mMSCs have the poten—tial to differentiate into insulin—producing cells when a combination of PDX-1(pancreatic and duodenal home—obox—1),NeuroDl(neurogenic differentiation-1)and MafA(V-maf musculoaponeurotic fibrosarcoma oncogenehomogog A)genes are transfected into them and expressed in these cells。Results：Insulin biosynthesis and seere—tion were induced in mMSCs into which these three genes have been transfected and expressed。The amount ofinduced insulin in the mMSCs which have been tranfected with the three genes together is significantly higherthan in those mMSCs that were only transfected with one or two of these three genes.Transplantation of the trans—feeted cells into mice with streptozotoein—induced diabetes results in insulin expression and the reversal of theglucose challenge.Conclusions：These findings suggest major implications for cell replacement strategies in gen—eration of surrogate β-cells for the treatment of diabetes.