【摘 要】
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Objectives In vitro studies showed fibroblast growth factor-2 (FGF-2) controls migration of mesenchymal stem cells (MSCs)In the present study,we established concentration gradient of FGF-2 between cor
【机 构】
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Beijing Anzhen Hospital, Capital Medical University, Beijing
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Objectives In vitro studies showed fibroblast growth factor-2 (FGF-2) controls migration of mesenchymal stem cells (MSCs)In the present study,we established concentration gradient of FGF-2 between coronary venous blood and target myocardium by coronary venous retroperfusion and assessed the effects of FGF-2 gradient on in vivo homing and differentiation of MSCs.Methods Acute myocardial infarction (AMI) was induced by ligation of left anterior descending (LAD) coronary artery.First,twelve animals were randomized into 4 groups based on the time of balloon dilation (retroperfusion time),being 0 min (n=3),5 min (n=3),10min (n=3),15min (n=3).One week after AMI,FGF-2 was retrogradely perfused into anterior interventricular vein in parallel to LAD,and FGF-2 concentrations of serum and myocardial tissue were measured by ELISA.The time of FGF-2 gradients after coronary venous retroperfusion was evaluated.Second,ten animals were randomized to MSCs group (n=5) and FGF-2+MSCs group (n=5) after AMI.MSCs were cultured and transfected by pLEGFP-N1 vector before transplantation.One week after retroperfusion,EGFP-positive endothelial cells and cardiomyocytes in infarcted myocardium were compared between the two groups by immunofluorescence method.
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