【摘 要】
：
[Aim] Garlic-derived compounds have been widely used for their medicinal purposes to overcome the poor prognosis of ovarian cancer.In this study,we investig
【机 构】
：
CollegeofPharmaceuticalScience,ZhejiangChineseMedicalUniversity,Hangzhou,Zhejiang310022,P.R.China;Ca
【出 处】
：
2013抗肿瘤药物研究新进展与肿瘤个性化药物治疗论坛
论文部分内容阅读
[Aim] Garlic-derived compounds have been widely used for their medicinal purposes to overcome the poor prognosis of ovarian cancer.In this study,we investigated the antiproliferative and apoptosis-inducing effects of S-allylcysteine (SAC), a water-soluble garlic derivative, on human ovarian cancer cell line A2780.[Methods] Colony forming assay,Hoechst 33258 stain,Annexin V/PI staining, transwell invasion,wound healing and western blot assays were performed to analyze the SAC treated A2780 cells.[Results] We found that the proliferation of A2780 cells was inhibited and the apoptosis of A2780 was induced by SAC in a time-dependent manner.In addition,SAC remarkably reduced the migration of A2780 cells.Meanwhile, SAC potently downregulated the protein expression of pro-caspase 3,Parp-1 Bcl-2 and upregulated the expression of active-caspase 3 and Bax.Furthermore,SAC remarkably decreased the protein expression of Wnt5a,pAKT,c-Jun,which were key proteins in proliferation and metastasis.[Conclusion] SAC,as a promising naturally-derived drug candidate, suppresses the proliferation and induces apoptosis of ovarian cancer A2780 cells.
其他文献
目的：观察“截敏祛风汤”对变应性鼻炎大鼠的治疗作用，探讨其作用机制。
方法：选择健康大鼠40只，随机分为“截敏祛风汤”组、西替利嗪组、模型对照组和正常对照组，每组10只。
目的：探讨癌痛规范化治疗中癌痛患者在本院疼痛门诊的镇痛药物治疗疗效及毒副作用临床分析研究.方法：2012年4月16日起本院疼痛门诊首诊.研究自2012年4月16日至2013年4月15日疼
目的：探讨半导体激光不同功率、不同时间照射及照射不同时间后犬声带热损伤的程度，为临床应用激光治疗声带病变提供实验依据。
方法：选用实验犬为实验对象，半导体激光功率分
疼痛是肿瘤最常见的相关症状之一.癌痛或癌症相关性疼痛与非恶性肿瘤相关性疼痛对患者的影响有所不同.约1/4新诊断恶性肿瘤的患者,1/3正在接受治疗的患者以及3/4晚期肿瘤患者
目的：探讨核苷酸还原酶、切除修复交叉互补组基因1和乳腺癌易感基因在外周血中表达及与吉西他滨联合铂类治疗非小细胞肺癌疗效的关系.方法：荧光实时定量PCR检测晚期NSCLC患者肿
目的：观察艾迪注射液联合GP方案化疗治疗晚期非小细胞肺癌临床疗效.方法：2008年1月至2010年12月收治晚期非小细胞肺癌101例,随机分为艾迪注射液联合GP方案的化疗组(治疗组)49例
Evidence has been proposed tumor-associated macrophages (TAMs) are participated in the prognosis of tumor.Though hypoxia has been proved to play a prominent
[Aim] Chemotherapy-induced ROS not only contribute to apoptosis,but also trigger autophagy.Since autophagy is reported to protect cancer cells from apoptosi
Camptothecin(CPT) nanosuspension is prepared by anti-solvent precipitation combined with sonication with TPGS as stabilizer in our study to improve its solu
目的：制备姜黄素聚乙二醇-聚己内酯纳米粒(CUR-PEG-PCL-NPs),考察其在大鼠体内药动学特征,并研究其体外抑瘤作用.
方法：以聚乙二醇-聚己内酯(PEG-PCL)为载体,采用乳化溶剂