Virus-induced gene silencing (VIGS) is a RNA-mediated post-transcriptional gene silencing mechanism that protects plants against foreign gene invasion.In addition to allowing a better understanding of how plants defend themselves against plant viruses,VIGS has emerged as an extremely powerful functional genomics tool for knocking out gene expression of target plant genes in some plants.We used Tobacco rattle virus (TRV) derived VIGS vectors expressed from binary vectors within Agrobacterium to induce RNA silencing in Nicotiana benthamiana.Leaf infiltration is the most common method of agroinoculation used for VIGS but this method has limitations since it is laborious for large scale screening and some plants are difficult to infiltrate.