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The accelerated levels of carbon monoxide(CO)in the atmosphere from incomplete combustion of carbon based fuels activate CO oxidizers in the microbiology community.Metagenomics analysis has been applied to identity these microbial profiles.Reads from the hypervariable V6 region in the bacterial 16s rDNA were aligned and finally clustered into operational taxonomic units(OTUs).The OTUs from different stages in anaerobic condition with CO were classified.Alphaproteobacteria,Clostridia,Betaproteobacteria and Actinobacteria were the most abundant groups,while Alphaproteobacteria,Betaproteobacteria and Actinobacteria were variable groups.