论文部分内容阅读
Background The normal vaginal microflora is disrupted in bacterial vaginosis.This study aim to identify the organisms in vaginal flora associated with bacterial vaginosis by bacteria specific 16S rRNA-based analysis.Methods Vaginal swabs were collected from 91 bacteria vaginosis women and 82 healthy women who were diagnosed by Amsel criteria and vaginal smears Gram stain.Bacteria DNA was extracted from the swabs, and 16S rRNA sequences were evaluated by polymerase chain reaction for the presence of Mycoplasma hominis, the lactobacilli Lactobacillus iners and L.crispatus, and the anaerobes Gardnerella vaginalis, Atopobium vaginae, Mobiluncus curtisii, M.mulieris, Prevotella, Bacteroides fragilis, Leptotrichia/Sneathia spp.and Megasphaera.Results G.vaginalis (63.7%), Prevotella (63.7%) and A.vaginae (51.6%) were the organisms most frequently identified in the vaginal swabs of women with bacteria voginosis, whereas L.crispatus (61%), L.iners (52.4%), and G.vaginalis (45.1%) were most frequently identified in healthy ones.Anaerobes other than B.fragilis were more frequently detected in vaginal flora of bacteria vaginosis cases than in healthy cases (P < 0.05, odds ratio > 1.0).L.iners, M.hominis, and B.fragilis were detected with similar frequency in the two groups (P > 0.05).Conclusion Our findings indicate that G vaginalis, Prevotella, A.vaginae, M.curtisii, M.mulieris, Leptotrichia, and Megasphaera are associated with bacteria vaginosis.