【摘 要】
:
疫霉菌是一类重要的植物病原菌,其引起的疫病经常对农作物造成严重的危害.由大豆疫霉(Phytophthora sojae)引起的根腐病是一种对大豆破坏性极强的病害,其危害面积大、破坏程度重,在全球范围内每年造成的经济损失高达十几亿美元.最近我们在大豆疫霉中鉴定到一类新型病原相关分子模式(Pathogenassociatedmolecular patterns,PAMP),命名为XEG1.我们发现沉默
【机 构】
:
南京农业大学植物保护学院,江苏南京210095 Center for Genome Researc
论文部分内容阅读
疫霉菌是一类重要的植物病原菌,其引起的疫病经常对农作物造成严重的危害.由大豆疫霉(Phytophthora sojae)引起的根腐病是一种对大豆破坏性极强的病害,其危害面积大、破坏程度重,在全球范围内每年造成的经济损失高达十几亿美元.最近我们在大豆疫霉中鉴定到一类新型病原相关分子模式(Pathogenassociatedmolecular patterns,PAMP),命名为XEG1.我们发现沉默XEG1 的大豆疫霉转化子对大豆的侵染能力显著下降,表明XEG1 是大豆疫霉行使毒性功能所必须的.另外XEG1 可以被大豆、烟草等多种植物识别,诱导一系列的植物免疫反应包括活性氧迸发、胼胝质的积累及抗病相关基因的表达,并造成植物细胞坏死.进一步的功能分析发现该蛋白N-端一个34 个氨基酸的PXN1 功能域是诱导植物免疫反应的主要区域.XEG1 属于糖苷水解酶12 家族,具有木葡聚糖酶和β-葡聚糖酶的活性.通过质谱分析,在大豆中鉴定到XEG1 的一个结合蛋白Gly900.Gly900 具有抑制XEG1 糖苷水解酶活性的功能,并且可抑制大豆疫霉对大豆的侵染.XEG1 类型糖苷水解酶在多种疫霉、病原真菌和细菌中广泛存在,并且可被大豆、烟草、辣椒和番茄等多种植物识别,表明植物中存在一种保守的对该类模式分子的识别机制.植物对XEG1的识别依赖于细胞膜受体蛋白BAK1,但不依赖于任何已知的植物模式识别受体.因此,进一步分析植物中识别XEG1的模式受体及识别信号通路元件对认识植物对疫病菌的先天免疫机理、挖掘和利用植物广谱持久抗病资源具有重要理论和实践价值.
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