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5. The construction of recombinant heparinase Ⅱ efficient expression system in E.coli and analysis of r

The construction of recombinant heparinase Ⅱ efficient expression system in E.coli and analysis of r

来源 :The 7th Sino-US Conference of Chemical Engineering(第7届中美化学工程 | 被引量 : 0次 | 上传用户：caesarm4

【摘 要】

：

Heparinase Ⅱ is an important polysaccharide lyase capable of depolymerizing heparin and heparan sulfate,which has a wide use in heparin industry,but the main bottleneck for its industrial application

【作 者】

：

Nan Su Jingjun Wu Mei Li Ye Li Xiang Mei Chong Zhang Xinhui Xing 

【机 构】

：

Key Laboratory for Industrial Biocatalysis of Ministry of Education,Institute of Biological Engineer

【出 处】

：

The 7th Sino-US Conference of Chemical Engineering(第7届中美化学工程

【发表日期】

：

2013年11期

【关键词】

：

heparinase Ⅱ fusion protein single amino acid mutant thermal stability increase 

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　　Heparinase Ⅱ is an important polysaccharide lyase capable of depolymerizing heparin and heparan sulfate,which has a wide use in heparin industry,but the main bottleneck for its industrial application is lack of the effective expression system and improving the enzyme thermostability which causes the high enzyme cost.

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