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Non-coding RNA (ncRNA) genes make transcripts as same as the encoding genes,and ncRNAs directly function as RNAs rather than serve as blueprints for proteins.As the function of ncRNA is closely related to organllie genomes,it is desirable to explore ncRNA function by confirming its provenance.Based on the experimental data,a ncRNAs dataset with less than 80% sequence identity (ncRNA_361)[1]for different organelle genomes is built from NONCODE v3.0[2].The ncRNA sequences from the four kinds of organllie genomes are recognized by using the improved K-minimum increment of diversity classifier (iK-MID) algorithm based on the features from the topology secondary structure and reading frames.