Metabonomics is the branch of science concerned with the quantitative understandings of the metabolite complement of integrated living systems and its dynamic responses to the changes of both endogenous factors (such as physiology and development) and exogenous factors (such as environmental factors and xenobiotics).As a holistic approach,metabonomics detects,quantifies and catalogues the time related metabolic processes of an integrated biological system,ultimately,relates such processes to the trajectories of the physiological and pathophysiological events.Metabonomic characteristics of various human rumor tissues,such as human hepatocellular carcinoma and glioma,have been investigated using HRMAS NMR spectroscopy and multivariate data analysis to understand the molecular aetiology of carcinogenesis.Metabonomic changes associated with the cancer development process were studied in details and future applications of metabonomics in cancer researches are discussed.