藏丹参质量标准研究

来源 :2013全国中药与天然药物高峰论坛暨第十三届全国中药和天然药物学术研讨会 | 被引量 : 0次 | 上传用户:zhongxuanshiye
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  AIM To establish the quality standard for Salvia castanea Diefs f.tomentosa Stib..METHODS Salvia castanea Diefs f.tomentosa Stib.Were identified by Microscopic and TLC;HPLC was adopted to determine Tanshinone ⅡA (C19H18O3)and Cryptotanshinone(C19H2003), The separation was carried out on Sunfire-ODS C18 column, The mobile phases was the methanol-water80: 20), The wavelength was at 270nm.RESULTS The established identification methods were proper and reflection a good specificity.The experiment showed good linear relationship at the ranges of 0.04186 ~ 0.8372μg(r=0.9999) for Tanshinone ⅡA, 0.01722~0.3444μg(r=0.9999) for Cryptotanshinone.In addition,the average recoveries were 99.12%(RSD=l.55%),98.29%, RSD=0.91%.CONCLUSION The method is simple,accurate ,reliable and can be used for the determination of Aristolochic acid A.
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