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Toll-like receptor1(TLR1) is the important sensor that activated immune response by forming a heterodimer with TLR2 during microbial infection in mammals.This study reports the identification and characterization of a full-length orthologue of grass carp TLR1, and its mRNA regulation in bacterial and viral irffeetion.The cloned grass carp TLR1 (gcTLR1) cDNA exhibited 2920 bp in length and contains a 267-bp 5untranslated region (UTR), a 2382-bp open reading frame and a 271-bp 3UTR.