The frog(Pelophylax nigromaculata)and the big toad(Bufo gargarizans)are typical amphibian`s species.In this paper,the surface morphology and structural features of the frog and the big toad swere observed by stereo microscope(SM)and scanning electron microscope(SEM).The differences and similarities of the surface morphology and the microstructure in different parts between the frog and the big toad were contrasted and analysised.The mechanical properties of the body surface of the frog and the big toad were measured by using the nanoindentation systerm.The difference of nanomechanical properties of various parts of the body surface were comparied between the frog and the big toad.The results show that the holding time have no significant effect on the elastic modulus and the nano hardness.The influence of the loading force and the loading rate were limited to the elastic modulus and the nano hardness,based on the load and the indentation depth curve.It was found that there existed the creep effects.