【摘 要】
：
The Rhizopus Chinesis 12 can produce a kind of new plasmin that is similar to natokinase.Through single factor analyses and orthogonal experiment,the best medium were obtained,bran water 4%,glucose 0.
【机 构】
：
College of Food Science, South China Agricultural University, Guangzhou 510642, Guangdong, CHINA ;De
论文部分内容阅读
The Rhizopus Chinesis 12 can produce a kind of new plasmin that is similar to natokinase.Through single factor analyses and orthogonal experiment,the best medium were obtained,bran water 4%,glucose 0.3%,peptone 0.75% and tryptone 0.75.The optimal culture condition is obtained by orthogonal experiment also,the seed age 12h,volume of inoculation 3%,the initial pH 5.0~6.0,the volume of medium in 1000ml flask 80ml,shaking speed 170r/min,culture temperature 28~30℃ and culture time 60h.
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