【摘 要】
：
In addition to a fast activating and immediately inactivating inward sodium current, many types of excitable cells possess a noninactivating or slowly inactivating component: the persistent sodium cur
【机 构】
：
Institute of Neuroscience;The Fourth Military Medical University,Xi'an 710032,P.R.China
【出 处】
：
The 3rd Ion Channel Conference: Ion channels-Structure, Func
论文部分内容阅读
In addition to a fast activating and immediately inactivating inward sodium current, many types of excitable cells possess a noninactivating or slowly inactivating component: the persistent sodium current (INaP).The INaP is found in normal primary sensory neurons where it is mediated by tetrodotoxin-sensitive sodium channels.The dorsal root ganglion (DRG) is the gateway for ectopic impulses that originate in pathological pain signals from the periphery.However, the role of INaP in DRG neurons remains unclear, particularly in neuropathic pain states.Using in vivo recordings from single medium-and large-diameter fibers isolated from the compressed DRG in Sprague-Dawley rats,we show that local application ofriluzole, which blocks the INaP, also inhibits the spontaneous activity of A-type DRG neurons in a dose-dependent manner.Significantly, riluzole also abolished subthreshold membrane potential oscillations (SMPOs), although DRG neurons still responded to intracellular current injection with a single full-sized spike.In addition, the INnP was enhanced in medium-and large-sized neurons of the compressed DRG, while bath-applied riluzole significantly inhibited the INaP without affecting the transient sodium current (INaT).Taken together, these results demonstrate for the first time that the INaP blocker riluzole selectively inhibits INaP and thereby blocks SMPOs and the ectopic spontaneous activity of injured A-type DRG neurons.This suggests that the INaP of DRG neurons is a potential target for treating neuropathic pain at the peripheral level.
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