【摘 要】
：
目的：SIX1是一种转录因子,其负责调控细胞的生长等生物学行为.最近,Six1被报道涉及参与各种癌症发生发展,而其在胃癌中的意义尚不清楚.本研究旨在探讨Six1在胃癌中的临床意义和生物学功能.方法：采用免疫组化分析,研究SIX1在91例胃癌标本中的表达情况.质粒转染低内源性SIX1的SGC-7901,而在高内源性Six1的HGC-27细胞系中转染siRNA进行基因敲出.通过MTT法和集落形成法对S
【机 构】
：
中国医科大学附属第一医院,胃肠肿瘤外科,沈阳,110001
【出 处】
：
第9届全国胃癌学术会议暨第二届阳光长城肿瘤学术会议
论文部分内容阅读
目的：SIX1是一种转录因子,其负责调控细胞的生长等生物学行为.最近,Six1被报道涉及参与各种癌症发生发展,而其在胃癌中的意义尚不清楚.本研究旨在探讨Six1在胃癌中的临床意义和生物学功能.方法：采用免疫组化分析,研究SIX1在91例胃癌标本中的表达情况.质粒转染低内源性SIX1的SGC-7901,而在高内源性Six1的HGC-27细胞系中转染siRNA进行基因敲出.通过MTT法和集落形成法对Six1增殖的作用进行了评估.结果：Six1在23例(25.3％)胃癌标本中呈阳性表达,与浸润深度和肿瘤分化程度呈现正相关.在研究的胃癌细胞系中,Six1也中观察到的呈现高表达.在胃癌细胞中Six1呈高表达,抑制其表达会降低细胞增殖率和集落形成能力.Matrigel侵袭实验表明,Six1能够提高细胞侵袭的能力.此外,我们表明,Six1的表达激活Akt信号通路,并且上调细胞周期蛋白Cyclin D1,CDK4和CDK6.结论：研究证明Six1在胃癌的发生发展中起到关键作用,作为一个临床相关的蛋白可为胃癌的治疗提供新的靶点.
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