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目的探讨乙型肝炎病毒DNA和前S2抗原与HBVDNA含量之间的关系。方法采用ELISA法和荧光定量PCR分别检测了404例乙型肝炎患者血清中前S2抗原与HBVDNA含量。结果404例乙型肝炎患者HBVDNA含量(拷贝/ml)与前S2抗原阳性的关系随血清HBVDNA含量的减低,前S2抗原检出率则明显减少。168例HBeAg阳性血清中,HBVDNA、前S2抗原阳性检出率均很高,三者之间有很好的一致性。比较HBVDNA和前S2抗原在各型乙肝患者血清中的检出率,急性乙型肝炎组与其他各组比较均有显著差异(P<0.01)。结论乙肝病毒前S2抗原和乙肝病毒复制及传染性有密切的关系,从基因水平证实HBeAg是反映HBV复制的传染性的良好指标。定量测定HBVDNA和前S2抗原,是一种检测HBV感染有价值的血清学指标,对监测乙型肝炎病情发展具有重要的意义。
Objective To investigate the relationship between hepatitis B virus DNA and pre-S2 antigen and HBVDNA content. Methods Serum levels of pre-S2 antigen and HBVDNA in 404 patients with hepatitis B were detected by ELISA and fluorescence quantitative PCR respectively. Results The relationship between HBVDNA content (copy / ml) and the positive rate of pre-S2 antigen in 404 patients with hepatitis B was significantly decreased with the decrease of serum HBVDNA content. 168 cases of HBeAg-positive sera, HBVDNA, pre-S2 antigen positive detection rate were high, there is a good agreement between the three. Comparing the detection rate of HBVDNA and pre-S2 antigen in the serum of various types of hepatitis B patients, the acute hepatitis B group had significant difference compared with other groups (P <0.01). Conclusion Pre-S2 antigen of hepatitis B virus is closely related to the replication and infectivity of hepatitis B virus. HBeAg is a good indicator reflecting the infectiousness of HBV replication from the gene level. Quantitative determination of HBVDNA and pre-S2 antigen, is a valuable serological detection of HBV infection serological indicators, monitoring the development of hepatitis B is of great significance.