2. 您的位置
3. 首页
4. 会议论文
5. Molecular Beacon based Aptazyme for Amplified Fluorescence Detection of Adenosine

Molecular Beacon based Aptazyme for Amplified Fluorescence Detection of Adenosine

来源 :第八届全国化学生物学学术会议 | 被引量 : 0次 | 上传用户：zhenlic0300

【摘 要】

：

Aptazyme is a kind of nucleic acid probes which take advantage of the specific recognition of aptamer and the highly catalysis efficiency of DNAzyme.Here, using the elements of 8-17 DNAzyme, adenosine

【作 者】

：

Yong He Jin Huang Xiaohai Yang Kemin Wang 

【机 构】

：

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Enginee

【出 处】

：

第八届全国化学生物学学术会议

【发表日期】

：

2013年2期

【关键词】

：

DNAzyme Aptamer Molecular beacon Adenosine 

下载到本地 , 更方便阅读

下载此文 赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

Aptazyme is a kind of nucleic acid probes which take advantage of the specific recognition of aptamer and the highly catalysis efficiency of DNAzyme.Here, using the elements of 8-17 DNAzyme, adenosine aptamer and molecular beacon (MB), we developed two types of novel strategies for adenosine detection.

其他文献

铁椤枝叶中的含氮柠檬苦素类成分及其生物活性研究

楝科植物是天然界中柠檬苦素类成分主要来源,到目前为止,已从楝科植物中发现超过1160个各种类型的柠檬苦素类化合物,它们具有杀虫、昆虫拒食、抗微生物、抗病毒、抗肿瘤、抗炎等广泛生物活性.铁椤(Amoora tsangii (Merr.)X.M)为楝科崖摩属植物,是海南特有植物,在当地常用其树皮煎汁来祛虱.

会议

铁椤楝科γ-内酰胺柠檬苦素NF-κB

β-CD/1,2-DBE体系中氧氟沙星对映异构体的手性识别作用研究

手性药物在体内的药理活性、代谢过程及毒性存在着显著差异[1].因此,手性表征及识别在生命过程中极其重要.本文利用室温磷光光谱法以及时间分辨磷光光谱法实现了对氧氟沙星对映体的手性识别,同时,借助分子模拟等方法[2]进一步对分子手性识别机理进行了探讨.

会议

β-环糊精氧氟沙星手性识别

Synthesis and Biological Activity of Multi-targeting Platinum(Ⅱ) Anticancer Complexes

Multi-targeting anticancer drug that a molecule termed combi-molecule (C-molecule) with binary epidermal growth factor receptor (EGFR) targeting/DNA-damaging properties can overcome the limitations of

会议

multi-targeting anticancer drugsEGFRinhibitorsDNAplatinum

Converting a heme-transfer complex to genetically encoded FRET sensors for imaging heme in living ce

Heme is a prosthetic group for many essential proteins involved in diverse biological processes, but excess heme can be highly toxic to cells.There is currently a lack of direct and quantitative metho

会议

heme sensorFRETIsd system

Site-specific dual labeling of EGFR in living cells

Epidermal growth factor (EGF) receptor is a tyrosine kinase which regulates cell growth and differentiation and is implicated in many human cancers.In vivo site-specific labeling of fluorescent or fun

会议

EGFRdual labelingclick chemistrylipoic acid ligase

L-半胱氨酸尾式金属卟啉配合物对抗氰化物中毒药物研究

氰化物广泛用于生产氰乙酸、合成橡胶、塑料、油漆等民用过程当中,此外还容易成为不法(恐怖)分子实施犯罪的工具.接触这些氰化物容易引起中毒,且毒性高,伤害大,因此提供有效的急救和预防药物特别重要.

会议

L-半胱氨酸尾式金属卟啉配合物氰化物中毒救治

Direct electrochemistry of horseradish peroxidase based on calcium phosphate with hierarchical porou

Biomorphic calcium phosphate (CaP) nanostructures with hierarchical porous structure were successfully synthesized using natural porous cole pollen grains as templates and are further employed for hor

会议

calcium phosphateporousdirect electrochemistrybiosensor

硅钨酸对酪氨酸酶的抑制作用

测定市购硅钨酸(以下简写为SiW12)对蘑菇酪氨酸酶活性的抑制作用[1-3].结果表明,在pH 6.8的NaH2PO4-Na2HPO4缓冲液中,SiW12对酪氨酸酶有较强的抑制效果,其IC50=2.31 mmol/L.

会议

硅钨酸酪氨酸酶二酚酶活力抑制作用

Graphene/Polyaniline/gold nanoparticles nanocomposite for the direct electron transfer of glucose ox

A novel glucose biosensor was developed based on the direct electrochemistry of glucose oxidase (GOD) adsorbed in graphene/polyaniline/gold nanoparticles (AuNPs) nanocomposite modified glassy carbon e

会议

graphene/polyaniline/AuNPsglucose oxidasedirect electrochemistrybiosensor

利用LC-MS/MS对阿霉素酸敏前体药物的生物学特征的研究

利用LC-MS/MS方法比较了聚乙二醇-聚乳酸-阿霉素键合药(P(Dox))与阿霉素单体药(Dox)的体外释放,细胞摄取的研究.键合前体药物分别在pH=5.0,pH=6.5和pH=7.4三种醋酸胺缓冲溶液中对其有效药物分子阿霉素的释放速度进行测定,从试验结果看出键和药物在酸性溶液中的释放速率最快.pH=5.0时,24小时内释放总量的80％.

会议

阿霉素前体药LC-MS/MS酸敏性细胞吸收