【摘 要】
：
多囊卵巢综合征(PCOS)是以高雄激素血症、高胰岛素血症为主要内分泌特征的代谢紊乱综合征.至今发病机制不明,胰岛素抵抗导致的高胰岛素血症及其所引起的一系列病理生理变化仍是PCOS病因研究的重点.胰岛素受体(INSR)作为胰岛素作用的中间环节而备受人们关注.目前关于胰岛素受体卵巢等局部生殖器官的表达已有研究,但报道结论不一,胰岛素受体在局部生殖器官病理生理变化中的作用仍需要进一步探究,DNA甲基化是
【机 构】
：
广东省计划生育科学技术研究所,广东,广州,510000
【出 处】
：
广东省遗传学会第九届代表大会暨学术研讨会
论文部分内容阅读
多囊卵巢综合征(PCOS)是以高雄激素血症、高胰岛素血症为主要内分泌特征的代谢紊乱综合征.至今发病机制不明,胰岛素抵抗导致的高胰岛素血症及其所引起的一系列病理生理变化仍是PCOS病因研究的重点.胰岛素受体(INSR)作为胰岛素作用的中间环节而备受人们关注.目前关于胰岛素受体卵巢等局部生殖器官的表达已有研究,但报道结论不一,胰岛素受体在局部生殖器官病理生理变化中的作用仍需要进一步探究,DNA甲基化是基因表观遗传修饰的重要方式,INSR基因在PCOS中是否存在着表观遗传学改变而影响INSR在组织中的表达,少有报道.本实验通过观察免疫组织化学法检测大鼠卵巢、子宫内膜组织INSR蛋白的表达与定位；通过MSP定性分析模型组与对照组大鼠血液、卵巢组织DNA INSR基因启动子区域的甲基化状态.免疫组织化学检测显示INSR定位表达于细胞膜,在卵巢间质及卵泡颗粒细胞、膜细胞、卵母细胞中均存在阳性表达,但在两组中卵泡及间质中的表达强度无明显差异(P＞0.05)；INSR在子宫内膜中存在着表达,在模型组子宫内膜间质及腺体中表达强度高于对照组(P＜0.05).MSP结果显示模型组与对照组血液的DNA样本均检出部分甲基化条带、无甲基化条带,而在卵巢组织DNA中检出部分甲基化、完全甲基化、无甲基化条带,但两组之间的甲基化率无明显差异(P＞0.05).结果提示,PCOS大鼠模型中INSR在卵泡及间质表达无明显改变,INSR表达量可能与卵泡发育障碍及卵巢间质增生无明显相关性；INSR在子宫内膜中表达有显著差异,推测INSR可能与子宫内膜增生有相关性；卵巢组织INSR基因甲基化程度不同,可能与PCOS异质性有一定相关性,仍需要进一步研究.
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