In order to expand the genetic basis of Brassica napus germplasm,this article investigated the feasibility of creating novel germplasm in rapeseed by cruciferous interspecific hybridization.Results implied that podding ratio and crossing compatibility index differed among different species.In both interspecific hybridizations of Brassica napus and Brassica juncea,Brassica rapa and Brassica juncea,the podding ratios(95.0%and 97.5%)and crossing compatibility index(6.51 and 5.66)were much higher when Brassica juncea were used as maternal than those in reciprocal crosses.In the interspecific hybridization of Brassica napus and Brassica rapa,the podding ratio(97.5%)and crossing compatibility index(14.66)when Brassica napus was used as maternal were much higher than that of its reciprocal cross.By interspecific hybridization between Brassica juncea and Brassica napus,the character of fiddle-shaped leaf in Brassica juncea was transferred to Brassica napus,which was controlled by one pair of recessive genes.In this study,Brassica napus line with high olein acid content(83.44%)and low linolenic acid content(2.14%)were obtained.These novel Brassica napus germplasm with high olein acid content and low linolenic acid content also had low erucic acid content and low glucosinolates and could be directly used in production.