During the past decades,fast urbanization development in China broke into large amount of farmland and caused metal contamination in outskirts soil of cities.Crops cultivated on the polluted soil are affected so that not only their growth is restrained but also quality of fruits of crops fell down.Vegetation stresses diagnose and quality assessment exclusively based on plant sampling and analysis are often insufficient and expensive.Hyperspectral data can provide ample information to detect subtle reflectance variations that dues to elevated contamination levels because spectral information possesses of high number of bands and narrow bandwidth.This paper describes characteristics of rice canopy hyperspectral reflectance and investigates possible application of this information to estimate Pb concentration in paddy plants.Rice (Xian_you 63) was cultivated in the barrels averagely filled with 10.0 kg soil sampled from Jintan City of Jiangsu Province.In this experiment,Pb of 0 mg,2.5*207.2 mg,5.0*207.2 mg (signed by Pb0,Pbl and Pb2 respectively) was appended into per 1000 g soil as Pb(CH3COO)2.Together 5 young plants were shifted into every barrel.During 5 growth stages of the seeding,early tillering,full tillering,jointing and booting,rice canopy spectra were measured outside by mobile hyperspectral radiometer (ASD FieldSpec Pro FR,Analytical Spectral Devices,Inc) between 10:00 and 14:00 to ensure optimal sun condition.Before collecting spectra,the measurement was standardized by a BaSO4 panel.To reduce noise level,every measurement was recorded as the average of 10 consecutively acquired spectra.After spectra measurement,canopy leaves in the field-of-view of radiometer were divided into two groups in the laboratory.The first ones were scissor for the chlorophyll extraction by the solution mixed equally by acetone (100%) to ethanol (100%).Chlorophyll contents were mensurated by Model 721 spectrophotometer respectively.The second ones were dried,ground and dissolved by HC104-HNO3-H2SO4 for Pb mensuration by atomic absorption spectrophotometer (SpectraAA-220FS).Before relationship between spectral reflectance,chlorophyll content and Pb concentration was established by a simple linear regression model,mean comparison and T-test were made to investigate data difference significance and its variance source.In the course of 5 observations,rice canopy spectrum differentiates from each other significantly,especially the region of 560 nm and 690 nm in the stage of seeding and early tillering (α≤0.05).Therefore,it provides good reference for the supposition that both stages of seeding and early tillering can be sensitive growth stages for hyperspectral monitoring Pb contamination on rice.In addition,ordinary differential equation was made to find red edge position (R672 in this study) that VIs need.In this paper CNDPI (Normalized Difference Pollution Index) similar to NDVI to.investigate Pb concentrations changes of rice plants.CNDPI using R760,R672 and R552 fluctuates regularly at 5 growth stages like an "S" fell down.Distinct collinearity exists between Pb concentration and chlorophyll content in the linear regression analysis; however,by partial least squares (PLS) this disturbance can be eliminated to establish proper relationship between spectral information and Pb contamination levels.Results of the analysis of Pb-polluted rice canopy spectra indicate that hyperspectral data can be a potential technology for monitoring rice growth and quality affected by Pb.