【摘 要】
：
Objective: To investigate the potential role of p62, a characterized adaptor protein for selective autophagy, in RANKLinduced osteoclastogenesis.Methods: Real-time quantitative PCR and western blot an
【机 构】
：
School & Hospital of Stomatology,Wuhan University 237＃ Luoyu Road,Hongshan District,Wuhan,China
【出 处】
：
11th Asian Congress on Oeal and Maxillofacial Surgery(第十一届亚洲
论文部分内容阅读
Objective: To investigate the potential role of p62, a characterized adaptor protein for selective autophagy, in RANKLinduced osteoclastogenesis.Methods: Real-time quantitative PCR and western blot analyses were used to evaluate the expression levels of autophagy-related markers during RANKL-induced osteoclastogenesis in mouse macrophage-like RAW264.7 cells.Meanwhile, the potential relationship between p62/LC3 localization and F-actin ring formation was tested using double-labeling immunofluorescence.Then, the expression of p62 in RAW264.7 cells was knocked down using smallinterfering RNA (siRNA), followed by detecting its influence on RANKL-induced autophagy activation, osteoclast differentiation, and F-actin ring formation.
其他文献
目的:研究血管紧张素-(1-7) [Ang-(1-7)]预处理对脑组织缺血耐受能力的影响及相关机制.材料与方法:将250-280g雄性SD大鼠按随机数字表法分为8组,通过连接右侧侧脑室的微型渗透泵分别给予如下药物:组1:人工脑脊液;组2:Ang-(1-7)(1.1 1nmol/L,0.25μL/h);组3:Ang-(1-7)(1.1 1nmol/L;0.25 μ L/h)+A-779(Mas受体拮
目的:探讨亚剂量二甲双胍预处理对局灶性脑缺血的神经保护作用及相关机制.材料与方法:将250-280g雄性SD大鼠按随机数字表法分为6组,分别给予如下处理:组1:生理盐水(i.p.);组2:二甲双胍(10mg/mg,i.p.);组3:二甲双胍(10mg/mg i.p.)+生理盐水(i.p.);组4:二甲双胍(10mg/mg,i.p.) +Compound C(AMPK抑制剂;20mg/kg,i.p.
目的:探讨血清同型半胱氨酸(Homocysteine,Hcy)、巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)水平与颈动脉粥样硬化关系.方法:对162例住院和门诊的受试者,应用彩超观检测颈动脉,根据颈动脉超声检查结果分为3组:颈动脉内-中膜(IMT)正常组32例,颈动脉IMT增厚组39例,颈动脉斑块组91例.分别对各组受试者测定血清Hc
目的:观察延胡索乙素对小鼠坐骨神经慢性压迫性损伤所致神经病理性疼痛的镇痛作用以及对背根神经节Cav1.2表达的影响.方法:雄性C57BL/6小鼠30只,随机分为5组,分别为假手术组(S组)、CCI组(C组)、延胡索乙素组(L组).建立稳定的小鼠坐骨神经慢性损伤致神经病理性疼痛模型.按照神经病理性疼痛诱发和持续时间,又将L组分为诱导期组、诱导维持期组、全程低剂量组.诱导期组于疼痛诱导期(0-5天)、
Objective: The use of PM pedicle flap in the closure of intraoral or through and through defects after surgical excisions of oral malignancies has gained popularity, due to its versatility.The aim of
Objective: The size and specific shape of bone grafts are important for reconstruction of large mandibular defects.We wanted to repair large mandibular defects using bone engineered with allogenic man
Objective: To assess the application value of the free-style anterolateral thigh perforator flap (ALTPF) based on preoperative hand-held Doppler (HHD) probe for perforator location in headandneck reco
Objective: To compare ultrasonography (US), computed tomography (CT), and magnetic resonance imaging (MRI) for clinical differential diagnosis in patients with salivary gland tumor (SGT).Material and
Objective: To evaluate the outcomes and to identify the clinical characteristics of a recent cohort of patients with lymphoepithelial carcinoma of salivary gland.Methods: From January 2006 to January
Objective: To determine the pathological basis and clinical features of obstructive sialadenitis in transplanted submandibular glands (SMGs).Methods: A total of 161 patients (174 eyes) with keratoconj