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5. 睾丸支持细胞与大鼠海马细胞培养作用的初步研究

睾丸支持细胞与大鼠海马细胞培养作用的初步研究

来源 :中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the | 被引量 : 0次 | 上传用户：liuhuilei

【摘 要】

：

海马与学习、情绪、记忆等多种神经活动有关,应激能引起海马细胞受损,慢性应激选择性损害海马CA3区神经元,寻找海马细胞保护方法有重要意义。睾丸支持细胞可分泌多种生长因子,可能可以促进海马细胞的贴壁及生长。方法 新生SD乳鼠(出生48 h以内)的海马细胞分离培养,12-16天SD大鼠支持细胞分离培养,分为单纯DMEM组、支持细胞培养液组(SCM)、支持细胞直接共培养组,分别培养24 h、48 h、72

【作 者】

：

米美玲 邹挺 杨蓓 熊明娣 王晶磊 徐斯凡 

【机 构】

：

南昌大学医学院生理教研室,南昌330006 中央民族大学生命与环境科学学院,北京100081

【出 处】

：

中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the

【发表日期】

：

2007年12期

【关键词】

：

海马细胞 睾丸支持细胞 共培养 细胞生长 

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论文部分内容阅读

　　海马与学习、情绪、记忆等多种神经活动有关,应激能引起海马细胞受损,慢性应激选择性损害海马CA3区神经元,寻找海马细胞保护方法有重要意义。睾丸支持细胞可分泌多种生长因子,可能可以促进海马细胞的贴壁及生长。方法 新生SD乳鼠(出生48 h以内)的海马细胞分离培养,12-16天SD大鼠支持细胞分离培养,分为单纯DMEM组、支持细胞培养液组(SCM)、支持细胞直接共培养组,分别培养24 h、48 h、72h、96 h、120 h、144 h、168 h、240 h观察神经细胞数量和平均突起个数,对支持细胞共培养组采取甲苯氨兰特殊染色观察。所有结果采用方差分析的多重比较得出。

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