微小RNA-101抑制自噬对骨肉瘤细胞增值及凋亡的影响

来源 :第十六届全国骨肿瘤学术会议 | 被引量 : 0次 | 上传用户:wl349682847
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  目的:探讨微小RNA-101(miR-101)抑制骨肉瘤细胞自噬对骨肉瘤细胞增殖及凋亡的影响。方法:采用实时荧光定量PCR(RT-PCR)检测miR-101 在人骨肉瘤细胞系MG63 细胞和成骨细胞中的相对表达,经脂质体转染法将miR-101 模拟物(Mimics)及阴性对照转染MG63 细胞,并设立空白对照。
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