Objective To determine the full-length genome sequences of hepatitis delta virus (HDV) from the sera of a HDV RNA-positive subjects using reverse transcription polymerase chain reaction (RT-PCR).Method Two pairs of primers within the conserved regions were designed to amplify overlapping fragments of HDV cDNA,and then the two fragments were joined together through a unique Sal Ⅰ restriction enzyme site.We can get the full-length HDV cDNA by sequencing the clones,After that we blast the sequence with other HDV genome.Result The unit-length HDV genome was 1 677bp,and the sequence homogeneity with genotype Ⅰ、Ⅱ、Ⅲwere 86-98%、80-81% and 89%,separately.Conclusion The whole HDV genome can be cloned by reverse transcription polymerase chain reaction.The analysis of the sequence revealed the sample used in this experiment was genotype Ⅰ.