【摘 要】
：
Environmental hormone belongs to a class of chemicals in the environment.It can affect the endocrine system of human and animals and lead to the reproductive, developmental and behavioural abnormaliti
【机 构】
：
Department of Public Health in Tianjin Medical University, Tianjin 300070, China
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
Environmental hormone belongs to a class of chemicals in the environment.It can affect the endocrine system of human and animals and lead to the reproductive, developmental and behavioural abnormalities, phthalate esters(PAEs)are one of the important environmental hormones, mainly used as plasticizers.In this paper, we choosed 5 phthalate monoesters which were reported greater toxicity as research objects, including MMP, MEP, MBP, MBzP and MEHP.Five Phthalate metabolites were extracted from the urine sample using liquid-liquid extraction and the amounts of urine were 20ml.The Optimization conditions of liquid-liquid extraction were that the usage amount of n-hexane was 10 ml and three times.Then flowed the elution solution to dry by nitrogen, and diluted them to 200μl with pure water.The contents of 5 kinds of phthalates metabolites were determined using RT-HPLC with the wave-length of determine was 228nm(Chart 1).HPLC condition: Sepax Technologies GP-Phenyl(5μm,4.6×250mm,120A) cartridge; acetonitrile(0.2 % acetic acid): water(0.2 % acetic acid)=45∶55 (v/v) as mobile phase.
其他文献
In recent years, liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) technique has been widely applied to analyze amino acids in biological fluids with protein precipitation or
Nanoflow liquid chromatography/mass spectrometry (nano-LC/MS), which has been developed from 1990s, generates increasing interest because it offers high detection sensitivity, reduces sample consumpti
Nanomaterials based chemosensors for ultrasensitive sensing strategies are becoming increasingly important in modem environmental and food sciences1.In our laboratory, we will present some designed ch
Capillary electrochromatography (CEC) is a hybrid technique combing capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) with high efficiency, high speed and good selectivi
With the widespread environmental pollution of pesticides, novel methods of decontamination and clean-up are recommended.Owing to the relatively high cost and the non-specificity of conventional techn
Ionic liquids (ILs) has been an attractive research subject of stationary phases for GC due to its peculiar properties.Our group proposed a practical approach to prepare the stationary phase of polysi
An ultra fast liquid chromatography/ion trap-time of flight mass spectrometry (UFLC/MS-IT-TOF) based serum lipidomics platform was developed to characterize the serum lipid profile of patients with Ch
Dye-sensitized solar cells (DSSC) are currently attracting significant interest as next-generation photovoltaics because of their low cost and high efficiency.To obtain high conversion efficiency, res
Headspace sampling coupled with gas chromatography (HS-GC) is a widely used technique for testing VDK and acetaldehyde in beer throughout the world.Sharing the headspace sampling, the two analyses are
Perkin Elmers SwaferTM micro-channel flow technology is an innovative and user-friendly approach for flow-switching applications-it delivers unparalleled hardware and application flexibility, expandin