【摘 要】
：
Background:Porcine circovirus type 2 (PCV2) is associated with postweaning multisystemic wasting syndrome in pigs.A monoclonal antibody (mAb) 8E4 againstthe PCV2 capsid protein has the capacity to neu
【机 构】
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Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology,Harbin Veter
【出 处】
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2013国际猪繁殖与呼吸综合征学术研讨会
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Background:Porcine circovirus type 2 (PCV2) is associated with postweaning multisystemic wasting syndrome in pigs.A monoclonal antibody (mAb) 8E4 againstthe PCV2 capsid protein has the capacity to neutralize the virus.However,this mAb can only react with some PCV2a strains (LG,CL,and JF2;mAb 8E4-positive strains),but does not cross-react with some PCV2b strains (YJ and JF;mAb 8E4-negative strains).Former experiments demonstrated that one amino acid substitution,alanine (A) for arginine (R) at position 59 (A59R) in the capsid protein of PCV2a (CL,LG,and JF2) strains,completely inhibited the reactivity of three PCV2a strains with mAb 8E4.However,an R→A substitution at position 59 (R59A) in the capsid protein of PCV2b/YJ did not induce recognition or neutralization by mAb 8E4.The objective of this study was to investigate which aa change(s) in the capsid protein of PCV2b (YJ and JF) strains caused the switch from mAb 8E4-negative to mAb 8E4-positive.
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