Fluoroquinolone is commonly used antibiotics to the patients with acute prostatitis because of broad spectrum of action, good bioavailability, and high concentrations in prostatic tissue.However, the incidence of quinolone-resistant microbes have been increased from 15.2％ in 2002 to 22.5％ in 2012 in Korea.The objectives of this study were to investigate the ciprofloxacin resistance and the prevalence of extended-spectrum beta-lactamases (ESBLs).We reviewed the medical records of 262 patients who were diagnosed acute prostatitis between January 2007 and December 2014.The uropathogens were isolated in 103 of the total 262 patients.Of the 103 patients, 82 patients were classified to community acquired group and 21 patients to hospital acquired group.We determined the ciprofloxacin resistance and the prevalence of ESBLs.Of the 82 patients classified to community acquired group, the most commonly isolated bacterium were Escherichia coli with a frequency rate of (65.9％) followed by Enterococcus faecalis (19.5％), Klebsiella pneumonia (4.9％), and Psudominas aeruginosa (2.4％).Of the 21 patients classified to hospital acquired group, the isolated bacterium were Escherichia coli (47.6％), Enterococcus faecalis (14.3％), Klebsiella pneumonia (23.8％), and Pseudomonas aeruginosa (4.8％).The ciprofloxacin resistance rate of community acquired group were documented for E.coli (7.3％), E.faecalis (1.1％), K.pneumonia (1.1％).For hospital acquired group, the resistance rate of E.coli, E.faecalis, and K.pneumonia were 33.4％, 9.5％, and 9.5％, respectively.Of total 103 patients, the prevalence of ESBLs were 7.8％.The prevalence by pathogen were 1.9％ of E.coli and 1.0％ of K.pneumonia in community acquired group and 3.0％ and 1.0％ in hospital acquired group, respectively.Despite of increasing FQ resistance, ciprofloxacin is effective to patients with community acquired acute prostatitis.However, patients with hospital acquired acute prostatitis need other antibiotics or combination therapy.