【摘 要】
：
该研究以亚洲薄荷中的青薄荷为试材,主要从选取合适的外植体,筛选最佳激素配比的培养基,寻找良好培养环境以缩短生产周期及瓶外生根以提高移栽成活率这几个实现薄荷再生植株
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该研究以亚洲薄荷中的青薄荷为试材,主要从选取合适的外植体,筛选最佳激素配比的培养基,寻找良好培养环境以缩短生产周期及瓶外生根以提高移栽成活率这几个实现薄荷再生植株构建产业化生产的关键处进行了研究,结果表明:薄荷的不同器官外植体在建立无菌培养体系中,茎段及茎尖在丛生芽的诱导和外植体生长上效果明显,启动率分别达100%和89.32%,适合建立无菌系及试管苗的快速繁殖中应用,而叶片不能诱导分化出芽,不宜作外植体.培养基的不同激素水平对薄荷茎段的生长分化有显著影响:6-BA在0.5-5 mg/L范围内有利于茎段芽的诱导,浓度提高,诱导芽数增加;NAA在0.05-1mg/L范围内苗的高生长明显,在0.05-0.3mg/L有利于根的诱导.激素不同水平组合试验结果表明对薄荷茎段芽的诱导及生长质量要优于单激素培养基,并筛选出最有利于芽的诱导及生长的培养基为MS+6-BA2mg/L+NAA0.1mg/L,增殖系数达7.7.
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