【摘 要】
：
糖苷水解酶是一类具有重要意义的酶。α-甘露糖苷酶(α-Man)和p-氨基己糖苷酶(β-Hex)是生物界广泛存在的糖苷水解酶。本研究通过已公布的甜瓜基因组数据库预测出8个α-Man和
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糖苷水解酶是一类具有重要意义的酶。α-甘露糖苷酶(α-Man)和p-氨基己糖苷酶(β-Hex)是生物界广泛存在的糖苷水解酶。本研究通过已公布的甜瓜基因组数据库预测出8个α-Man和3个β-Hex基因家族成员,进行生物信息学分析,并且克隆CmMAN2和CmHEX1基因的cDNA序列。甜瓜CmMAN2基因全长1538bp,编码512个氨基酸。CmHEXl基因全长1666bp编码,505个氨基酸。不同发育时期果实酶活性检测表明,授粉天数0天时α-Man和β-Hex活性要显著高于10-40天。不同组织中的检测结果表明,叶中表达量最高。实时荧光定量PCR分析结果显示,CmMAN2和CmHEXl基因在甜瓜不同时期的果实中和在甜瓜不同组织中均有表达。CmAN2在40天的果实中表达量最高,而cmHEXl在0天和20天的果实中表达量最高。在不同组织的检测中,CmMAN2在茎中表达量最高,CmHEXl在叶中表达量最高。
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