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目的通过向大鼠脑缺血损伤区的靶向移植转化生长因子-β1(TGF-β1)基因修饰的神经干细胞,观察TGF-β1基因修饰的神经干细胞对大鼠脑缺血损伤的治疗作用。方法建立大鼠的局灶性脑缺血再灌注模型,并随机分为假手术组(A组)、磷酸盐缓冲液PBS组(B组)、神经干细胞治疗组(C组)、TGF-β1基因修饰神经干细胞组(D组),根据取样时间点的不同每组又分为3、7及14 d共三组(每组5只),手术后3、7、14 d进行神经功能评分(NSS),采用5-溴-2,-脱氧尿嘧啶核苷(BrdU)标记处于增殖状态的细胞,应用免疫组化法检测BrdU阳性细胞。结果术后3、7、14 d C组和D组NSS评分明显低于A组和B组,统计学分析差异有统计学意义(P<0.05),其中D组NSS评分较C组低,两组比较差异有统计学意义(P<0.05);C组和D组大鼠在脑缺血/再灌注后3、7、14 d均可检测到BrdU阳性细胞,BrdU阳性细胞计数显示各个时间点C组和D组阳性细胞数明显多于对照组(P<0.05)。结论在局灶性脑缺血再灌注模型大鼠损伤区注入TGF-β1基因修饰的神经干细胞,对脑缺血所致的损伤具有一定的修复作用。
Objective To observe the therapeutic effect of transforming growth factor-β1 (TGF-β1) gene modified neural stem cells (TGF-β1) modified neural stem cells on cerebral ischemia injury in rats by targeted transplantation into ischemic area of rats. Methods The model of focal cerebral ischemia-reperfusion in rats was established and randomly divided into sham operation group (group A), phosphate buffered saline group (group B), neural stem cell treatment group (group C), TGF-β1 According to the different sampling time points, each group was divided into 3 groups (5 rats in each group) at 3, 7 and 14 days, and neurological function score was evaluated at 3, 7 and 14 days after operation NSS). BrdU-positive cells were labeled with 5-bromo-2-deoxyuridine (BrdU), and BrdU positive cells were detected by immunohistochemistry. Results The NSS scores of C group and D group were significantly lower than those of A and B groups at 3, 7 and 14 days after operation (P <0.05). The NSS score of D group was lower than that of C group (P <0.05). BrdU positive cells were detected in both groups C and D at 3, 7 and 14 days after cerebral ischemia / reperfusion, and the number of BrdU positive cells showed that at each time point The number of positive cells in group C and group D was more than that in control group (P <0.05). Conclusion The injection of TGF-β1 gene-modified neural stem cells into the lesion area of focal cerebral ischemia-reperfusion model rats can repair the damage induced by cerebral ischemia.