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目的研究人胚胎中枢神经系统ＮＯＶｍＲＮＡ神经元的发育。方法地高辛标记的ｃＲＮＡ探针原位杂交技术。结果（１）脊髓：第１６周人胚胎脊髓腹侧出现ＮＯＶｍＲＮＡ神经元，为大型神经元，至第２８周扩展到后角中间神经元，第３８周中央灰质中阳性神经元明显。（２）延髓：第１６周延髓观察到下橄榄核、舌下神经核、迷走神经背核均有阳性神经元分布，至第２８周，三叉神经脊束核和楔束副核检测到阳性神经元；（３）脑桥、中脑和间脑：第３２周脑桥展神经核、中脑红核和黑质、丘脑腹外侧核和背内侧核均有阳性神经元分布。（４）大脑：第３２周纹状体有较多标记神经元，第３８周大脑皮质顶叶阳性神经元明显。以上结果表明ＮＯＶｍＲＮＡ神经元在人胚胎中枢神经系统中，首先在低级中枢出现，在高级中枢中逐渐发育；各胎龄标本中所观察到的阳性神经元多位于支配躯体运动的神经核团；随着胚胎中枢神经系统的发育，ＮＯＶｍＲＮＡ神经元逐渐增多，这与其他原癌基因在胚胎发育的早期表达高，而后降低不同。结论在人胚胎发育过程中ＮＯＶ基因对神经系统的分化和进一步的功能活动可能起重要调节作用，且这种作用逐渐增强。
Objective To study the development of NOV mRNA neurons in central nervous system of human embryo. Methods Digoxigenin labeled cRNA probe in situ hybridization. Results (1) Spinal cord: The NOV mRNA neurons appeared in the ventral part of human embryonic spinal cord at the 16th week, which were large neurons. The neurons in the central gray matter extended to the posterior horn neurons at the 28th week. (2) Medulla oblongata: Positive neurons were found in the medulla oblongata, sublingual nucleus and vagal dorsal nucleus in the 16th week. By the 28th week, the positive neurons ; (3) Pons, midbrain and diencephalon: There were positive neurons in the pontine nucleus, midbrain red nucleus and substantia nigra, dorsal ventromedial nucleus and dorsal medial nucleus in the 32nd week. (4) The brain: There were more labeled neurons in the striatum on the 32nd week, and the parietal lobe positive neurons on the 38th week were obvious. The above results show that NOV mRNA neurons in the central nervous system of human embryos first appeared in the lower center and developed gradually in the higher center. The positive neurons observed in each gestational age mostly located in the nucleus that dominated somatic movement. With the development of the embryonic central nervous system, the number of NOV mRNA neurons gradually increases, which is higher than that of other proto-oncogenes during the early embryo development and then decreases. Conclusion The NOV gene may play an important regulatory role in the differentiation of nervous system and further functional activity in human embryonic development, and this effect is gradually enhanced.