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目的:探讨匹罗卡品(PILO)诱发的癫痫大鼠模型脑组织中神经肽Y(Neuropeptide Y,NPY)含量的动态变化及意义,进一步明确NPY与癫痫的关系,为抗癫痫治疗,研制抗癫痫药物提供新途径。方法:健康成年雄性SD大鼠120只,随机分为两组:单纯腹腔注射匹罗卡品组(癫痫模型组);单纯腹腔注射生理盐水组(对照组);注射后根据Racine制定的标准判定是否有癫痫发作,并行脑电图检查,观察有无癫痫样波(棘波,尖波,棘慢波,尖慢波)发放。两组大鼠分别于给药后1h,3h,6h,24h,3d,7d,15d,30d,60d将大鼠麻醉,取出脑组织,对脑组织中的NPY含量进行测定。结果:癫痫模型组60只大鼠中,2只死于癫痫持续状态,其余大鼠可观察到边缘发作行为表现,脑电图有典型的癫痫样波发放,对照组无癫痫发作及癫痫样波发放。癫痫模型组脑NPY含量与对照组相比,差异有显著性P<0.05;癫痫模型组急性期(1h-7d)与慢性期(15d-60d)比较差异有显著性P<0.05,对照组差异无显著性;癫痫模型组与对照组脑中的NPY含量在12h,24h,15d,30d,60d.差异有显著性P<0.05或P<0.01,癫痫模型组脑中的NPY含量各组(各时间段)比较有差异有显著性P<0.05,对照组差异无显著性,癫痫模型组大鼠Ⅳ-Ⅴ级发作与Ⅱ-Ⅲ级发作,脑NPY含量比较,差异有显著性P<0.05。结论:1.神经肽Y与癫痫密切相关,癫痫发作后?
Objective: To investigate the dynamic changes and significance of neuropeptide Y (NPY) in the brain tissue of pilocarpine induced epilepsy rat models. To further clarify the relationship between NPY and epilepsy, to develop anti-epilepsy Epilepsy drugs provide a new way. Methods: One hundred and twenty healthy adult male Sprague-Dawley rats were randomly divided into two groups: Pilocarpine group (epilepsy model group), intraperitoneal injection of normal saline group (control group), according to the standard of Racine Whether there is seizures, parallel electroencephalography, epilepsy-like waves observed (spikes, spikes, spikes and slow waves, sharp waves slow) release. The rats were anesthetized at 1h, 3h, 6h, 24h, 3d, 7d, 15d, 30d and 60d after administration, respectively. The brain tissues were removed and the content of NPY in the brain tissues was determined. Results: Two of the 60 rats in the epilepsy model group died of status epilepticus. The performance of the peripheral seizures was observed in other rats. The typical epileptiform wave was distributed in the EEG group. There was no epileptic seizure and epileptiform wave in the control group Issued. Compared with control group, the content of NPY in epilepsy model group was significantly lower than that in control group (P <0.05); the difference between epilepsy model group in acute phase (1h-7d) and chronic phase (15d-60d) was significant NPY content in the brain of epilepsy model group and control group at 12h, 24h, 15d, 30d, 60d.The difference was significant P <0.05 or P <0.01, NPY content in the brain of epilepsy model group (P <0.05). There was no significant difference in the control group. The level of Ⅳ-Ⅴ and the level of NPY in the epilepsy model group were significantly different (P <0.05). Conclusion: 1. Neuropeptide Y and epilepsy are closely related to seizures?