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目的探讨子宫内膜癌组织抑癌基因PTEN及p53异常表达的意义。方法采用免疫组织化学SP法检测62例子宫内膜癌、16例子宫内膜增生过长和9例正常子宫内膜组织PTEN蛋白及P53蛋白的表达情况,表达率的差异采用卡方检验。结果子宫内膜癌、子宫内膜增生过长和正常子宫内膜组的PTEN蛋白表达缺失率分别为64.52%、50%和11.11%,子宫内膜癌和子宫内膜增生过长组明显高于正常子宫内膜组(P<0.01),但前两组间差异不显著;子宫内膜癌组、子宫内膜增生过长和正常子宫内膜组的P53蛋白阳性表达率分别为46.77%、50%和55.56%,三组间无显著差异(P>0.05);PTEN蛋白表达缺失和P53蛋白阳性表达与子宫内膜癌组织分化程度无相关关系(P>0.05);在子宫内膜癌组中,PTEN蛋白表达缺失组P53蛋白阳性表达率为37.50%,显著低于PTEN阳性表达组的63.64%(P<0.05)。结论PTEN蛋白在子宫内膜癌的发生中可能起着比P53蛋白更为重要的作用。
Objective To investigate the significance of abnormal expression of tumor suppressor gene PTEN and p53 in endometrial carcinoma. Methods Immunohistochemical SP method was used to detect the expression of PTEN protein and P53 protein in 62 cases of endometrial carcinoma, 16 cases of endometrial hyperplasia and 9 cases of normal endometrium. The difference of expression rate was analyzed by chi-square test. Results The loss of PTEN protein expression in endometrial carcinoma, endometrial hyperplasia and normal endometrium were 64.52%, 50% and 11.11%, respectively, which were significantly higher than that in endometrial carcinoma and endometrial hyperplasia Normal endometrium group (P <0.01), but there was no significant difference between the two groups. The positive rates of P53 protein in endometrial carcinoma group, endometrial hyperplasia group and normal endometrium group were 46.77%, 50 % And 55.56%, respectively. There was no significant difference between the three groups (P> 0.05). There was no correlation between the expression of PTEN protein and the expression of P53 protein in endometrial carcinoma (P> 0.05) The positive expression rate of P53 protein in the PTEN-deficient group was 37.50%, which was significantly lower than that in PTEN-positive group (63.64%, P <0.05). Conclusion PTEN protein may play a more important role than P53 protein in the pathogenesis of endometrial carcinoma.