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目的:了解本院鲍曼不动杆菌中I类整合子的分布与基因盒结构,并探讨整合子与鲍曼不动杆菌耐药的关系。方法:收集鲍曼不动杆菌临床株,用纸片扩散法(K-B法)测定77株鲍曼不动杆菌对18种抗生素的敏感性,采用PCR法检测鲍曼不动杆菌Ⅰ类整合酶基因(intI1)并扩增其可变区,对PCR产物进行酶切分析,并测序分析整合子可变区携带的耐药基因盒。结果:鲍曼不动杆菌耐药现象十分严重。77株菌株中有49株含Ⅰ类整合子,阳性率63.64%,其中有45株(91.84%)整合酶阳性株扩增出可变区,共检测出4种耐药基因盒组合形式:aacA4(0.8kb)、dfrXII-orfF-aadA2(1.8kb)、aacA4-catB8-aadA1(2.3kb)、aacC1-orfX-orfX-orfX’-aadA1a(3.0kb)。整合子阳性菌株与整合子阴性菌株对多种抗生素耐药性存在差异。结论:Ⅰ类整合子与鲍曼不动杆菌耐药性密切相关,在多重耐药鲍曼不动杆菌耐药机制中起重要作用。
OBJECTIVE: To understand the distribution and gene box structure of class I integrons in Acinetobacter baumannii and to investigate the relationship between integron and Acinetobacter baumannii. Methods: The clinical strains of Acinetobacter baumannii were collected and the susceptibility of 77 strains of Acinetobacter baumannii to 18 kinds of antibiotics was determined by disk diffusion method (KB method). The Acinetobacter baumannii integrase gene (intI1) was amplified and its variable region was amplified. The PCR products were digested with restriction endonucleases and sequenced. Results: The Acinetobacter baumannii resistance was very serious. Among the 77 strains, 49 strains contained class I integron, with a positive rate of 63.64%. Among them, 45 strains (91.84%) of integrase positive strains amplified variable regions, and four combinations of resistant gene cassettes were detected: aacA4 (0.8 kb), dfrXII-orfF-aadA2 (1.8 kb), aacA4-catB8-aadA1 (2.3 kb), aacC1-orfX-orfX-orfX’-aadA1a (3.0 kb). Integron-positive strains and integron-negative strains of a variety of antibiotic resistance differences. CONCLUSION: Class I integrons are closely related to the drug resistance of Acinetobacter baumannii and play an important role in the drug resistance mechanism of Acinetobacter baumannii.