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采用ＢａｍＨＩ酶切和琼脂糖凝胶电泳技术，从重组质粒ＰｓＦｌｌ中回收５ｋｂ小鼠白血病病毒（ＭｕＬＶ）ＤＮＡ片段，经光敏生物素标记后制备ＤＮＡ探针，以斑点杂交法检测杂交瘤细胞、ＭｃＡｂ纯品以及ＳＰ２／０细胞中的ＭｕＬｖ。结果表明：此探针灵敏度可达２５ｐｇ，特异性好，在被检测的１３株杂交瘤细胞和２株ＳＰ２／０细胞中分别查出５株和１株为ＭｕＬｖ阳性，ＭｃＡｂ纯品中未发现ＭｕＬｖ。
A 5kb DNA fragment of murine leukemia virus (MuLV) was recovered from the recombinant plasmid PsF11 by BamHI digestion and agarose gel electrophoresis. DNA probes were prepared by labeling with biotin and hybridoma cells were detected by dot blot hybridization. Pure and MuLv in SP2 / 0 cells. The results showed that the sensitivity of this probe was up to 25 pg and the specificity was good. Five of the 13 hybridomas and two SP2 / 0 cells tested were positive for MuLv, but no McAb was found MuLv.