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建立了测定人血清中头孢克罗的高效液相色谱法。分析柱为Ｓｈｉｍ－ｐａｃｋＣＬＣ－ＯＤＳ（５μｍ），６．０ｍｍ×１５０ｍｍ；流动相为磷酸盐缓冲液（ｐＨ５．８）∶乙腈＝８７．５∶１２．５，流速为１．２ｍＬ／ｍｉｎ；紫外检测波长为２５４ｎｍ。血清中头孢克罗浓度在０．１２５～２４ｍｇ／Ｌ范围内，峰高与浓度呈良好的线性关系，回归方程为Ｈ＝－３．２００＋９２．２９Ｃ（ｎ＝９，ｒ＝０．９９９９），日内、日间变异系数分别为４．１％和５．８％，平均回收率为９９．３％。
A HPLC method was established for the determination of cefaclor in human serum. The analytical column was Shim-pack CLC-ODS (5 μm), 6.0 mm × 150 mm; the mobile phase was phosphate buffer (pH 5.8): acetonitrile = 87.5: 12.5 and the flow rate was 1.2 mL / min; Detection wavelength of 254nm. The concentration of cefaclor in serum ranged from 0.125 to 24 mg / L, the peak height and concentration showed a good linear relationship, the regression equation was H = -3.200 + 92.29C (n = 9, r = 0.9999) The intra-day and inter-day coefficient of variation were 4.1% and 5.8%, respectively. The average recovery rate was 99.3%.